Some of the criticisms look... odd. In particular, why just Primer-BLAST of all to check the primer sequences?
There are other tools out there for that kind of calculation, and to make sure it weren't an algorithm-specific problem they should also have used others.
Some of the criticisms look... odd. In particular, why just Primer-BLAST of all to check the primer sequences?
There are other tools out there for that kind of calculation, and to make sure it weren't an algorithm-specific problem they should also have used others.